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. 2022 Aug 8;18(13):5123–5135. doi: 10.7150/ijbs.66673

Figure 3.

Figure 3

The circARID1A directly bound to miR-370-3p. A. Schematic illustration of the seven binding sites for miR-370-3p in circARID1A. B. Images of FISH showed that circARID1A and miR-370-3p co-localized in the cytoplasm of U87 cells and GBM specimen, scale bar, 150μm (upper) 50μm (lower). C. Dual-luciferase reporter assay showed that co-transfection of WT1 and mimic miR-370-3p markedly decreased luciferase activity in 293T cells, whereas luciferase activity did not change in 293T cells when miR-370-3p binding sites in circARID1A were mutated. n = 3, *p < 0.05, t-test. D. Left: Images of agarose gels showed that biotin-labeled probe specifically targeted circARID1A, with oligo probe as a negative control; Right: Histogram of qPCR of RNA pull-down assay. Compared with oligo probe, miR-370-3p in the group probe was significantly enriched. n = 3, *p < 0.05, t-test.