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. 2022 Aug 8;18(13):5123–5135. doi: 10.7150/ijbs.66673

Figure 4.

Figure 4

The circARID1A promoted migration and invasion via modulation the miR-370-3p/TGFBR2 pathway in vitro. A. Data from GEO datasets (GSE25632/ GPL8179) indicated that hsa-miR-370 was significantly downregulated in GBM compared with NBT. *p < 0.05, t-test. B. Venn diagram for predicted targets of miR-370-3p by ENCORI. C. Dual-luciferase reporter assay showed that co-transfection of WT2 and mimic miR-370-3p markedly decreased luciferase activity in 293T cells, whereas luciferase activity did not change in 293T cells when miR-370-3p binding sites in circARID1A were mutated. n = 3, *p < 0.05, t-test). D. Images of agarose gel showed that biotin-labeled miR-370-3p pulled down circARID1A and TGFBR2, with oligo probe as a negative control. TGFBR2 and circARID1A were markedly abundant in group biotin-labeled miR-370-30. E. Left: Images of Transwell assays of U87 cells; Right: Analyses of Transwell assays results indicated that transfections of miR-370-3p inhibitor promoted U87 cell migration and invasion, while silencing circARID1A suppressed the promotion. scale bar, 50μm n = 5,*p < 0.05, ANOVA. F. Images of western blotting showed that expression of TGFBR2 protein was decreased after silencing circARID1A. G. Images of western blotting showed that transfections of miR-370-3p inhibitor promoted TGFBR2 expression, while silencing circARID1A suppressed the promotion.