REAGENT or RESOURCE	SOURCE	IDENTIFIER
Biological samples
N. sp. 295	USA: East Hawaii Island	EHBR
N. ogradyi	USA: East Maui Island	EMBR
N. kanawao	USA: North Hawaii Island	NHBR
N. makaihe	USA: West Oahu Island	WOBR
N. sp. 23	USA: Kauai Island	KICL
N. haleakala	USA: Haleakala Mtn, Maui	HMCO
N. sp. 302	USA: Hawaii Island	HICO
N. sp. 58	USA: Maui Island	MICO
N. sp. 29	USA: Kauai Island	KIDO
N. maratima	USA: Oahu Island	OIDO
N. sp. 281	USA: Hawaii Island	HIMY
N. sp. 126	USA: Kauai Island	KIMY
N. sp. 242	USA: Molokai Island	MIMY
N. sp. 246	USA: Oahu Island	OIMY
N. montium	USA: Hawaii Island	HIPI
N. sp. 17	USA: Kauai Island	KIPI
N. sp. 48	USA: Maui Island	MIPI
N. ponapona	USA: Oahu Island	OIPI
N. sp. 15	USA: Molokai Island	MIPS
N. sp. 21	USA: Kauai Island	KISC
Orosius sp.
Deposited Data
BioProject	NCBI – GenBank	PRJNA816609
Sulcia sequence data	NCBI – GenBank	CP093890 to CP093909
Nasuia sequence data	NCBI – GenBank	CP094180 to CP094197
Insect mitochondrial sequence data	NCBI – GenBank	ON135504 to ON135524
Software and algorithms
Trimmomatic	Bolger et al., 2014	v0.39
FastQC	Andrews, 2010	v0.11.9
SPAdes	Bankevich et al., 2012	v3.14
GLIMMER	Delcher et al., 2007	v3.02
Geneious Pro	Drummond et al., 2011
BLAST	Altschul et al., 1990
Bowtie2	Langmead and Salzberg, 2012	v2.3.5.1
RAST	Overbeek et al., 2014	v2
RNAmmer	Lagesen et al., 2007	v1.2
tRNAscan-SE	Lowe and Chan, 2016	v2.0
MITOS	Bernt et al., 2013
MAFFT	Katoh and Standley, 2013	v7.455
PartitionFinder 2	Lanfear et al., 2017
BEAST	Drummond and Rambaut, 2007	v.1.10.4
Tracer	Rambaut et al., 2018	v1.7.1
RWTY	Warren et al., 2017	v1.0.2
Phytools	Revell, 2012	v.1.0–1
RStudio	RStudio Team, 2018
MEGAX	Kumar et al., 2018	v.10.2.4
JModelTest2	Darriba et al., 2012	v2.1.10
PAML (codeml)	Yang 2007	v4.8
Other
Sulcia genome assembly from Macrosteles quadrilineatus	Bennett and Moran (2013)	NCBI: CP006060
Nasuia genome assembly from Macrosteles quadrilineatus	Bennett and Moran (2013)	NCBI: CP006059
Sulcia genome assembly from Entylia carinata	Mao et al., 2017	NCBI: CP021172
Nasuia genome assembly from Entylia carinata	Mao et al., 2017	NCBI: CP021173