Figure 1.

Administration of NMN increased NAD⁺ levels in mice challenged by LPS. (a) NAD⁺-biosynthesis salvage pathways and NAD⁺ catabolism. (b) The animal experimental flowchart. C57BL/6 mice (20–25 g) were subjected to different doses of NMN (100, 300, and 500 mg/kg/day) or PBS intraperitoneally at 18:00 daily for 7 days. LPS (15 mg/kg) or 0.9% normal saline were injected via caudal vein, respectively, after 1 h. Then, mice were sacrificed 12 h after LPS injection and lung tissues were collected. (c) Pulmonary total NAD⁺ contents and ratios of NAD⁺/NADH in mice. (d) Mitochondrial NAD⁺ contents and ratios of NAD⁺/NADH in mice in each group. (e) Nuclear NAD⁺ contents and ratios of NAD⁺/NADH in mice in each group. (f) The ratios of mitochondrial NAD⁺ contents/ nuclear NAD⁺ contents in mice in each group. Statistical difference was calculated by one-way ANOVA (n = 5/group). (A color version of this figure is available in the online journal.)