TABLE 2.
Strains used
| Strain | Genotype |
|---|---|
| L40 | MATa his3 trp1 leu2 ade2 LYS2::(lexAop)4-HIS3 URA3::(lexAop)8-lacZ GAL4) |
| TK60 | L40/pBTM116-LEXA-SKN7/pRS425 |
| TK61 | L40/pBTM116-LEXA-SKN7/pRS425-MID2 |
| SEY62102 | MATα/MATa leu2/leu2 ura3/ura3 his3/his3 lys2/lys2 trp1/trp1 suc2/suc2 |
| SEY6210 | MATα leu2 ura3 his3 lys2 trp1 suc2 |
| SEY6210a | MATa leu2 ura3 his3 lys2 trp1 suc2 |
| TK82 | SEY6210a/pRS426 |
| TK83 | SEY6210a/pRS426-MID2 |
| TK84 | SEY6210a/pRS426-MID2-HA |
| TK85 | SEY6210a/pRS426-ΔS/T-MID2-HA |
| TK86 | SEY6210a/pVT101U |
| TK87 | SEY6210a/pVT101U-MID2 |
| TK88 | SEY6210a mid2Δ::KANMX2 |
| TK89 | SEY6210a mid2Δ::KANMX2/pRS425 |
| TK90 | SEY6210a mid2Δ::KANMX2/pRS425-WSC1 |
| TK91 | SEY6210a mid2Δ::KANMX2/pRS425-WSC2 |
| TK92 | SEY6210a mid2Δ::KANMX2/pRS425-RHO1 |
| TK93 | SEY6210a mid2Δ::KANMX2/pFL44 |
| TK94 | SEY6210a mid2Δ::KANMX2/pFL44-MPK1-HA |
| TK95 | SEY6210a/pRS425 |
| TK96 | SEY6210a/pFL44 |
| TK97 | SEY6210a/pFL44-MPK1-HA |
| TK98 | SEY6210a/pRS426-MID2-GFP |
| TK99 | SEY6210a chs3Δ::LEU2/pVT101U |
| TK100 | SEY6210a chs3Δ::LEU2/pVT101U-MID2 |
| TK101 | SEY62102kre6Δ::HIS3/KRE6 mid2Δ::KANMX2/MID2 |
| TK102 | SEY62102kre9Δ::HIS3/KRE9 mid2Δ::KANMX2/MID2 |
| TK103 | SEY62102fks1Δ::HIS3/FKS1 mid2Δ::KANMX2/MID2 |
| TK104 | SEY62102wsc1Δ::KANMX2 mid2Δ::KANMX2/MID2 |
| TK105 | SEY62102mtl1Δ::GFP-HIS3 mid2Δ::KANMX2/MID2 |