TABLE 3.
Measurement of total cellular chitin
| Condition | Strain | nmoles of GlcNAc/mg (wt) of cells (mean ± SD) | Increase from control (nmol of GlcNAc/mg [wet wt] of cells) | MID2-dependent change (%) |
|---|---|---|---|---|
| A (YEPD [control]) | Wild type | 3.54 ± 0.06 | ||
| mid2Δ | 3.60 ± 0.31 | |||
| B (YEPD + calcofluor white [10 μg/ml], 2 h) | Wild type | 8.96 ± 0.16 | 5.42 | |
| mid2Δ | 6.82 ± 0.14 | 3.22 | −40 | |
| C (YEPD) | kre6Δ | 9.50 ± 0.43 | 5.96 (from wild type) | |
| kre6Δ mid2Δ | 7.90 ± 0.08 | 4.30 (from mid2Δ) | −28 | |
| D (YEPD + 4 μM α-factor, 3 h) | Wild type | 5.05 ± 0.19 | 1.51 | |
| mid2Δ | 3.91 ± 0.05 | 0.31 | −79 | |
| E (YNB [uracil dropout]) | Wild type carrying pVT101U | 4.65 ± 0.65 | ||
| Wild type carrying pVT101U-MID2 | 11.87 ± 0.22 | +255 |