FIG. 3.

Germination of mutant spores in the germinants l-alanine (A) and l-asparagine (B). Spores from wild-type strain PS832 (■) or mutant strain FB8 (mut4) (◊, ⧫), FB9 (mut8) (●), or FB10 (mutb1) (▴) were assayed for germination in buffer alone (open symbols), 10 mM l-alanine (closed symbols in panel A), or 10 mM l-asparagine (closed symbols in panel B) as described in the legend to Fig. 2. Note that d-glucose was not present in these germination reactions. Spores from all strains produced overlapping, reasonably flat curves when incubated in buffer alone, and only one representative curve is shown (◊ in both panels).