TABLE 3.
Effect of wild-type and mutagenized gerB operons on germination of wild-type spores
| Mutationa | MLSr transformants
|
|
|---|---|---|
| No. testedb | % Germinatedc | |
| None (no plasmid) | 174 | 0 |
| None (wild type) | 123 | 0 |
| Gly297 (GGU)→Ser (AGU) | 68 | 25 |
| Pro326 (CCA)→Ser (UCA) | 68 | 15 |
| Phe269 (UUU)→Ile (AUU) | 40 | 52 |
a
The mutations were introduced by site-directed mutagenesis into a plasmid-borne, otherwise wild-type gerB operon. Plasmid DNA (300 to 500 ng) was introduced into the wild-type strain (PS832) by congression with the MLS resistance marker of strain 1A640 (10 ng of chromosomal DNA).
b
By plate assay for d-alanine-induced germination.
c
Percentage that developed a red color in the plate assay for d-alanine-induced germination.