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. 2022 Aug 5;20:100473. doi: 10.1016/j.ynstr.2022.100473

Fig. 1.

Fig. 1

Anatomy of ventral hippocampal terminals in GAD and CRF reporter mice.

a) EYFP (green) in glutamatergic pyramidal cells in the injection site in the ventral hippocampus and subiculum using an AAV2 virus with CAMKII-promoter driven expression in a GAD-tdTomato (red) mouse brain with DAPI counterstain (blue). Section sliced in the parasagittal plane and annotated with outlines from Allen Brain Atlas (sagittal view 2/21). b) Strong expression of EYFP (green) was seen in axons coursing through the fornix (fx), both anterior and posterior of the anterior commissure (ac), in a GAD-tdTomato (red) mouse. c) Diagram of nuclei in parasagittal sections of this region (Allen Mouse Brain Atlas and Allen Reference Atlas - Mouse Brain, sagittal view 19/21 image credit: Allen Institute for Brain Science https://atlas.brain-map.org/atlas?atlas=2#atlas=2&plate=100883858). d) Numerous hippocampal terminals (green) in the medial regions of the BNST in close proximity to GAD + neurons (red). e) Sparse hippocampal terminals expressing ChR2-EYFP (green) near CRF + neurons in the PVN in a coronal section from a CRF-tdTomato (red) mouse. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)