FIG. 1.

TLC of maltose reaction products obtained by a dialyzed T. litoralis cell extract (A), 4-α-glucanotransferase-free cell extract (B), and purified 4-α-glucanotransferase (C). Dialyzed cell extract (1.4 mg of protein per ml), glucanotransferase-free cell extract (4.6 mg of protein per ml), or purified glucanotransferase (11 μg of protein per ml) was incubated with 10 mM maltose in 100 mM MOPS (pH 7.0) at 85°C. Aliquots of the reaction mixture (10 μl) were applied to the TLC plate at different time intervals. S, standards; G₁, glucose; G₂, maltose; G₃, maltotriose; G₄, maltotetraose.