FIG. 4.

TLC of ¹⁴C-labeled reaction products of purified 4-α-glucanotransferase with unlabeled maltose and [U-¹⁴C]glucose (A) or with unlabeled maltose and [U-¹⁴C]maltose (B). The reaction mixture (45 μl) contained 1.6 μg of enzyme, 10 mM unlabeled maltose in 100 mM MOPS (pH 7.0), and 0.33 nmol of [U-¹⁴C]glucose or 0.6 nmol of [U-¹⁴C]maltose. Aliquots of the reaction mixture (10 μl) were applied to the TLC plate at different time intervals. G₁, glucose; G₂, maltose; G₃, maltotriose; G₄, maltotetraose.