FIG. 5.

Temperature dependence of T. litoralis MalP activity. The assay mixture containing 2 μg of enzyme per ml in 50 mM potassium phosphate (pH 7.0)–5 mM maltoheptaose was incubated for 15 min. The enzyme activity was assessed by the production of glucose-1-phosphate as described in Materials and Methods. For all temperatures tested, glucose-1-phosphate production was linear with time.