TABLE 1.
Purification of 4-α-glucanotransferase from T. litoralis
Purification step | Total protein (mg) | Total activity (U)a | Sp act (U/mg)a | Yield (%) |
---|---|---|---|---|
Cell extract | 40 | 6.4 | 0.16 | 100 |
Amylose column | 0.059 | 1.5 | 25.7 | 23 |
4-α-Glucanotransferase activity was measured at 95°C with 10 mM maltotriose as the substrate.