TABLE 3.
Specific enzyme activities in cell extracts from T. litoralis grown with different carbon sources
| Carbon source | Activity (mU/mg)a
|
|
|---|---|---|
| MalPb | PNPG hydrolysisc | |
| Peptone | 48 | 17 |
| Tryptone | 51 | 16 |
| Yeast extract + peptone | 57 ± 8 | 16 ± 1 |
| Maltose + peptone | 88 ± 10 | 15 ± 1 |
| Dextrins + peptone | 108 ± 9 | 21 ± 3 |
a
Values for activities measured in extracts of cells grown in peptone or tryptone correspond to the averages of triplicate determinations in a single experiment. The others are averages of activities measured at least in three independent extracts.
b
Measured at 60°C with 5 mM maltoheptaose as the substrate, using the assay with coupled enzymes described in Materials and Methods.
c
Measured at 80°C with 1 mM PNPG.