Figure 2.

One i.p. injection with 4.5 mg/kg S1, 4.5 or 10 mg/kg GBZ transiently reduces eIF2α phosphorylation in 2b4 ^he 2b5 ^ho mice. Two‐and‐a‐half‐month‐old male early symptomatic 2b4 ^he 2b5 ^ho mice received one i.p. injection of 2.25% PEG300. 4.5 or 10 mg/kg GBZ. Mice were terminated 4 or 24 h after injection. Brains were taken out. Cerebella were lysed and samples were subjected to SDS‐PAGE and Western blot to detect Ser51‐phosphorylated eIF2α and total eIF2α. Graphs show individual and mean values, ±SD. Results were statistically analyzed with an unpaired t‐test. *p < 0.05. [Colour figure can be viewed at wileyonlinelibrary.com]