FIG. 1.
Schematic representation of the relationship between pIP823 from L. monocytogenes (3,712 bp), pUB110 from S. aureus (4,525 bp), and pTB913 from Bacillus stearothermophilus (4,525 bp). bleo, gene conferring resistance to bleomycin; dfrD, gene encoding trimethoprim-resistant S2DHFR; dso, double-stranded origin or plus origin of replication; knt, gene conferring resistance to kanamycin; mob, gene encoding the mobilization protein Mob; repU, gene encoding the replication protein RepU; RSA, palindromic site involved in conjugative mobilization and recombination; ssoU, single-stranded origin or minus origin of replication. The positions of the PCR primers in dfrD (D), mob (M), dso (R1), and repU (R2) are indicated by filled arrowheads.