TABLE 1.
Bacterial strains, plasmids, and transposons
| Strain, plasmid, or transposon | Relevant characteristic(s)a | Reference |
|---|---|---|
| Strains | ||
| Bacillus subtilis | ||
| BM4150 | Strr | 7 |
| 168 | trpC2 Lvs+ Prt+ | 17 |
| Escherichia coli | ||
| DH5α | F− (φ80ΔlacZΔM15) endA1 recA1 hsdR17 supE44 thi-1 λ− gyrA96 relA1 Δ(lacZYA-argF)U169 | 37 |
| HB101 | F−hsdS20 (rB− mB−) Δ(gpt-proA)62 leu supE44 λ− ara-14 galK2 lacY1 Δ(merC-mrr) rpsL20 (Strr) xyl-5 mtl-1 recA13 | 27 |
| JM83 | F−ara Δ(lac-proAB) rpsL (Strr) φ80lacZΔM15 | 38 |
| K802N | hsdR mutant, hsdM+ F−gal met supE Nalr Rifr | 27 |
| 14R525 | Prototroph, Nalr | 31 |
| SM10 | RP4-2-Tc::Mu Kmrthi thr leu suIII | 30 |
| Enterococcus faecalis | ||
| JH2-2 | Fusr Rifr, spontaneous mutant of JH2 | 14 |
| BM4110 | Strr, spontaneous mutant of JH2 | 7 |
| Listeria monocytogenes | ||
| BM4293 | pIP823 | 4 |
| LO17RF | Fusr Rifr, spontaneous mutant of LO17 | 4 |
| EGDSmR | Strr, spontaneous mutant of EGD | 4 |
| Staphylococcus aureus | ||
| RN4220 | Restriction-deficient derivative of 8325-4 | 16 |
| 80CR5RF | Rifr Fusr Res−, spontaneous mutant of 80CR5 | 10 |
| 80CR5Str | Strr Res−, spontaneous mutant of 80CR5 | 10 |
| Plasmids | ||
| pIP823 | Tpr (dfrD) Tra− Mob+ | 4 and this study |
| pUC18 | Plasmid vector, AprlacZα Tra− Mob− | 38 |
| pAT459 | pIP823 linearized with HindIII cloned into pUC18 | This study |
| pAMβ1 | Tra+ Emr | 5 |
| pAT191 | oriR pAMβ1 oriR pBR322 tra pAMβ1 oriT RK2 KmrlacZα | 34 |
| RP4 | Tra+ IncP Apr Kmr Tcr | 1 |
| R64 | Tra+ IncI1 Smr Tcr | 1 |
| pIP55-1 | Tra+ IncA-C Cmr Sur | 1 |
| pOX38-Km | Tra+ IncF1 Kmr | 2 |
| pBR322 | Tra− Apr Tcr | 1 |
| pSC101 | Tra− Tcr | 1 |
| Transposon Tn1545 | Tra+ Emr Kmr Tcr | 7 |
a
Cmr, resistance to chloramphenicol; Emr, resistance to erythromycin; Fusr, resistance to fusidic acid; Kmr, resistance to kanamycin; Nalr, resistance to nalidixic acid; Rifr, resistance to rifampin; Smr, plasmid-mediated resistance to streptomycin; Strr, chromosomal resistance to streptomycin; Sur, resistance to sulfonamide; Tcr, resistance to tetracycline; Tpr, resistance to trimethoprim.