Figure 2.

(A) Equilibrium binding assays with membranes prepared from HEK293 cells expressing the hA₃AR. Membranes were incubated with 0.3 nM [¹²⁵I]I-AB-MECA and 10 μM modulator compounds for 18 h. Data are presented as the percent change in specific binding compared to vehicle. (B) Dissociation binding assays with membranes prepared from HEK293 cells expressing the hA₃AR. Membranes were incubated with ∼0.3 nM [¹²⁵I]I-AB-MECA and 10 μM modulator compounds for 3 h when the assay was initiated by the addition of 100 μM NECA. After 60 min, the amount of specific binding remaining was determined. Data are presented as mean ± SEM. *p < 0.05 vs vehicle by Student’s t tests. Percent change from vehicle (A) and % [¹²⁵I]I-AB-MECA remaining after 60 min (B) values are reported in Tables S1 and S2.