Table 2. Peptides Used for the Affinity Resin/Proteomics Quantification of Active ADAMs and MMPs in Human IPF Samples and Human Healthy Lung Controlsa.
| protein | peptide sequence | Q1 precursor ion m/z | Q3 product ion m/z |
|---|---|---|---|
| ADAM9 | FLPGGTLCR | 510.77 [M + H]2+ | 606.30 [M + H]+ y5 663.32 [M + H]+y6 760.38 [M + H]+ y7 |
| DCFIEVNSK | 556.26 [M + H]2+ | 447.26 [M + H]+ y4 689.38 [M + H]+ y6 836.45 [M + H]+y7 | |
| FGNCGFSGNEYK | 690.29 [M + H]2+ | 844.38 [M + H]+ y7 901.41 [M + H]+ y8 1061.44 [M + H]+y9 | |
| ADAM10 | AIDTIYQTTDFSGIR | 850.93 [M + H]2+ | 579.32 [M + H]+ y5 896.45 [M + H]+ y8 1187.57 [M + H]+y10 |
| FSLCSIR | 441.73 [M + H]2+ | 535.27 [M + H]+y4 648.35 [M + H]+ y5 735.38 [M + H]+ y6 | |
| LVDADGPLAR | 513.78 [M + H]2+ | 456.29 [M + H]+ y4 699.38 [M + H]+ y7 814.41 [M + H]+y8 | |
| ADAM17 | GEESTTTNYLIELIDR | 927.46 [M + H]2+ | 758.44 [M + H]+ y6 871.52 [M + H]+y7 1034.59 [M + H]+ y8 |
| GYGIQIEQIR | 588.82 [M + H]2+ | 786.45 [M + H]+y6 899.53 [M + H]+ y7 956.55 [M + H]+ y8 | |
| AYYSPVGK | 442.73 [M + H]2+ | 487.29 [M + H]+ y5 650.35 [M + H]+y6 813.41 [M + H]+ y7 | |
| MMP-1 | VTGKPDAETLK | 579.82 [M + H]2+ | 773.4 [M + H]+y7 901.5 [M + H]+ y8 958.52 [M + H]+ y9 |
| LTFDAITTIR | 575.83 [M + H]2+ | 674.42 [M + H]+y6 789.45 [M + H]+ y7 936.51 [M + H]+ y8 | |
| DIYSSFGFPR | 594.79 [M + H]2+ | 710.36 [M + H]+ y6 797.39 [M + H]+ y7 960.46 [M + H]+y8 | |
| MMP-8 | QFMEPGYPK | 548.76 [M + H]2+ | 690.35 [M + H]+y6 821.39 [M + H]+ y7 968.45 [M + H]+ y8 |
| SISGAFPGIESK | 596.81 [M + H]2+ | 630.35 [M + H]+y6 777.41 [M + H]+ y7 848.45 [M + H]+ y8 | |
| YYAFDLIAQR | 630.32 [M + H]2+ | 600.38 [M + H]+ y5 862.48 [M + H]+ y7 933.52 [M + H]+y8 | |
| MMP-14 | VGEYATYEAIR | 636.32 [M + H]2+ | 752.39 [M + H]+ y6 823.43 [M + H]+y7 986.49 [M + H]+ y8 |
| VWESATPLR | 529.79 [M + H]2+ | 644.37 [M + H]+ y6 773.42 [M + H]+ y7 959.49 [M + H]+y8 | |
| EVPYAYIR | 505.77 [M + H]2+ | 685.37 [M + H]+ y5 782.42 [M + H]+y6 881.49 [M + H]+ y7 | |
| internal standard (yeast enolase) | TAGIQIVADDLTVTNPK | 878.48 [M + H]2+ | 772.46 [M + H]+ y7 1002.51 [M + H]+ y9 1172.62 [M + H]+y11 |
| VNQIGTLSESIK | 644.86 [M + H]2+ | 676.39 [M + H]+ y6 777.44 [M + H]+ y7 1075.60 [M + H]+y10 | |
| NVNDVIAPAFVK | 643.86 [M + H]2+ | 561.34 [M + H]+ y5 632.38 [M + H]+y6 844.53 [M + H]+ y8 |
a
For quantification, three peptides per proteinase were used. Precursor ions were selected in Q1 (first quadrupole) then fragmented in Q2, and the specified product ions were monitored in Q3. In bold are the fragments used for quantification.