Fig. 1.

Flow cytometric analysis of monocyte subpopulations. Freshly isolated peripheral blood mononuclear cells were stained and analysed for monocyte subpopulations. The pan-monocyte marker CD86 was used to identify all monocytes. Subsequently, 3 monocyte subsets were identified based on the patterns of CD16 and CD14 expression. The following cell definitions were used: classical monocyte: CD14⁺⁺CD16^–; intermediate monocyte: CD14⁺⁺CD16⁺; and non-classical monocyte: CD14⁺CD16⁺.