Fig. 1.

Downregulated circ_0009043 in NSCLC is associated with poor prognosis. A PCR product of cric_0009043 was detected in 1.5% agarose gel electrophoresis. B Representative images of circ_0009043 determined by RNA-FISH in NSCLC and normal tissues (× 200, scale bar 100 μm), respectively. C The expression level of circ_0009043 in that of NSCLC tissues along with normal lung tissues, n = 132. D Kaplan–Meier curve was plotted for the analyses of patients' survival rate on the ground of circ_0009043 expression. E Cellular localization of circ_0009043 in NSCLC cells was identified by employing RNA FISH while fractions of cytoplasmic and nuclear RNA were isolated and measured by performing RT-qPCR analysis. All results were representative of three separate experiments. Data represent mean values ± SD from three replicates of each sample; ****P < 0.0001