KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
α-INTS10	Abcam	Cat# ab180934s
α-INTS13	Bethyl	Cat# A303-575A; RRID: AB_11125549
α-INTS14	Prestige	Cat# HPA040651
α-C7orf26	Prestige	Cat# HPA052175
α-His	CST	Cat# 2366
α-Mouse	Licor	Cat# 926-32210
α-Rabbit	Licor	Cat# 926-32213
α-CD11b AF647	Biolegend	Cat# 101220; RRID: AB_493546
Bacterial and virus strains
MegaX Competent Cells	ThermoFisher	Cat# C640003
Stellar Competent Cells	Takara	Cat# 636766
Chemicals, peptides, and recombinant proteins
TransIT-LT1 Transfection Reagent	Mirus Bio	Cat# MIR2300
Critical commercial assays
Chromium Single-Cell 3ʹ v3 with Feature Barcoding	10x Genomics	PN-1000075, PN-1000153, PN-1000079
Seahorse XF Cell Mito Stress Test	Agilent	Cat# 103015-100
Bioanalyzer RNA nano	Agilent	Cat# 5067-1511
Deposited data
Raw sequencing data from Perturb-seq screens	This paper	SRA BioProject PRJNA831566
Processed data from Perturb-seq screens	This paper	http://gwps.wi.mit.edu
Experimental models: Cell lines
K562 dCas9-BFP-KRAB	Gilbert et al., 2014	N/A
RPE1 Zim3-dCas9	This study	N/A
Oligonucleotides
Dual sgRNA gDNA primer: AATGATACGGCGACCACCGAGATCTACACCGCGGTCTGTATCCCTTGGAGAACCACCT	Nunéz et al., 2021	oJR324
Dual sgRNA gDNA index primer: CAAGCAGAAGACGGCATACGAGATnnnnnGCGGCCGGCTGTTTCCAGCTTAGCTCTTAAA	Nunéz et al., 2021	oJR325
Custom R1 sequencing primer: CGCGGTCTGTATCCCTTGGAGAACCACCTTGTTGG	Nunéz et al., 2021	oJR326
Custom R2 sequencing primer: GCGGCCGGCTGTTTCCAGCTTAGCTCTTAAAC	Nunéz et al., 2021	oJR328
Custom Index Read 1 sequencing primer: GTTTAAGAGCTAAGCTGGAAACAGCCGGCCGC	Nunéz et al., 2021	oJR327
Recombinant DNA
See Table S9 for plasmids used in this study	This paper	N/A
Software and Algorithms
CellRanger 4.0.0	10X Genomics, Inc.	http://software.10xgenomics.com
sgRNA assignment scripts	Replogle et al., 2020	https://github.com/josephreplogle/guide_calling
Perturb-seq analysis codebase	Norman et al., 2019	https://github.com/thomasmaxwellnorman/Perturbseq_GI