FIG 2.

Gaps between nucleoids increased upon HBsu removal or inactivation. (A) HBsu depletion-degradation strain (BWX1340) was grown in the presence of 0.5 mM IPTG and then washed three times and resuspended in warmed medium containing 0.5% xylose for indicated times. FM4-64-stained membrane (red) and DAPI-stained nucleoids (green) are shown. Bar, 4 μm. (B) hbs(ts) (BWX4414) strain was grown at 30°C, shifted to 42°C, and imaged at the indicated time points. (C) dnaB(ts) (BWX2533) strain was grown at 30°C, shifted to 42°C, and imaged at the indicated time points.