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. 2022 May 12;204(8):e00119-22. doi: 10.1128/jb.00119-22

FIG 3.

FIG 3

Number of replication origins was reduced upon HBsu removal or inactivation. (A to C) Representative images of DAPI-stained nucleoids (blue), FM4-64-stained membrane (red), and replication origin (tetO/TetR-CFP, green) of cells growing under indicated conditions in CH medium. (A) HBsu depletion-degradation strain (BWX1340) was grown in the presence of 0.5 mM IPTG, washed three times, and resuspended in warmed medium containing 0.5% xylose. Bar, 4 μm. (B) hbs(ts) strain (BWX4414) was grown at 30°C, shifted to 42°C, and imaged at the indicated time points. (C) dnaB(ts) strain (BWX2533) was grown at 30°C, shifted to 42°C, and imaged at the indicated time points. (D) Analysis of number of origins per cell that contained nucleoids and the percentage of anucleate cells.