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. 2022 Aug 2;13:935692. doi: 10.3389/fimmu.2022.935692

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Copyright © 2022 Bahiraii, Brenner, Yan, Weckwerth and Heiss

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sfn does not affect Y105 phosphorylation but glutathionylation of PKM2 in M1 (LPS) macrophages. iBMDM were pretreated with DMSO or the indicated concentrations of Sfn for 30 min prior to stimulation with LPS for 6 h. (A) Total cell lysates were immunoblotted for p(Y105), total PKM2, and actin as a loading control. Representative blots of at least three independent experiments are depicted. Bar graphs represent compiled densitometric data. (B) Macrophages were pretreated with Sfn (10 μM) or the GSH donor glutathioneethylester (GSEE) before LPS was added for another 6 h. Proteins were harvested under nonreducing conditions. Lysates (input) or immunoprecipitates obtained with an antibody specific for protein-bound glutathione (GSS-X) were subjected to immunoblot analysis for PKM2. Representative blots of three independent experiments with consistent results are depicted.