Table 12 Phase 2 summary on the adequacy testing of existing guidance documents for the nutritional assessment. Per default the existing guidance refers to the case study Table 3, and if needed an additional guidance is mentioned

Case	Specific evaluations of the case, including intended and unintended effects	Conclusions on the adequacy of existing guidance	Updates recommended for future guidance
1	The product is a food enzyme, therefore nutritional assessment is not required.	Not applicable
2 and 3	The presence of XNA and xenoproteins in the product needs to be evaluated for nutritional and/or antinutritional consequences.	Not fully adequate	Updated guidance is recommended for assessing new‐to‐nature products
4	The nutritional assessment of the biomass of SynBio Aspergillus oryzae in soy sauce can be based on the comparison of the composition with the non‐GM counterpart with history of human consumption.	Adequate	No update needed
5	The nutritional assessment of the biomass of SynBio yeast cannot be based on the comparison of the composition with the non‐GM counterpart because it does not have a safe history of human and animal consumption as biomass.	Adequate	No update needed
6	The nutritional assessment of the biomass of SynBio cyanobacteria cannot be based on the comparison of the composition with the non‐GM counterpart because it does not have a safe history of human and animal consumption as biomass.	Adequate	No update needed
7–9	The engineered strains are designed to improve the nutritional and sensorial properties of food and feed. They are not expected to be nutritionally disadvantageous compared with the non‐GM parental strain that can be used as the non‐GM counterpart in the nutritional assessment.	Adequate	No update needed
10	The product is composed of bacteriophage for food decontamination, therefore nutritional assessment is not required.	Not applicable
11 and 12	The product is a feed additive, (category zootechnical additives), therefore, nutritional assessment is not required.	Not applicable
13	The product is a plant biostimulant, therefore, nutritional assessment is not required.	Not applicable
14	The product is a plant virus used as plant protection product, therefore nutritional assessment is not required.	Not applicable
15	The product is a gut bacterium used as feed additive, therefore nutritional assessment is not required.	Not applicable