TABLE 1.
Bacterial strains and plasmids used
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| Strains | ||
| E. coli XL1-Blue | F′::Tn10 proA+B+lacIq Δ(lacZ)M15 recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 | Stratagene |
| M. smegmatis mc2155 | Highly transformable mutant | 47 |
| M. smegmatis LGM1 | Tn611 insertion mutant of strain mc2155 | This work |
| M. aurum MO1 | Morpholine-degrading strain | 11 |
| Mycobacterium sp. | ||
| BM01 | Morpholine-, piperidine-, and pyrrolidine-degrading strain | 41 |
| BM04 | Morpholine- and pyrrolidine-degrading strain | 41 |
| BM05 | Morpholine- and pyrrolidine-degrading strain | 41 |
| BM06 | Morpholine- and pyrrolidine-degrading strain | 41 |
| FM10 | Morpholine- and pyrrolidine-degrading strain | 41 |
| FM30 | Morpholine- and pyrrolidine-degrading strain | 41 |
| LM20 | Morpholine- and pyrrolidine-degrading strain | 41 |
| LM32 | Morpholine- and pyrrolidine-degrading strain | 41 |
| LM40 | Morpholine- and pyrrolidine-degrading strain | 41 |
| RP1 | Morpholine-, piperidine-, and pyrrolidine-degrading strain | 40 |
| Plasmids | ||
| pBluescript II KS(+) | E. coli cloning vector; Ampr | Stratagene |
| pJQ200 | Cloning vector with sacB and aacC1 (Gmr) genes; nonreplicative vector in mycobacteria | 42 |
| pCG79 | Mycobacterium and E. coli shuttle plasmid, thermosensitive for replication or maintenance in mycobacteria, carries an Strr gene expressed in E. coli and the Tn611 transposon containing the Kanr gene from Tn903 which is expressed in mycobacteria and in E. coli | 19 |
| pLGM20 | pBluescript with 5.8-kb EcoRI fragment of M. smegmatis containing pipR, IS1096, pipA, orf1, and truncated orf2′ | This work |
| pLGM21 | pBluescript with 2,177-bp SacI-EcoRI fragment of pLGM20 | This work |
| pLGM22 | pLGM21 with a Kanr cassette in the blunt-ended MluI site | This work |
| pLGM23 | pJQ200 with XbaI-PstI fragment of pLGM22 in SmaI site | This work |