Author response image 2. MKK3 deletion attenuates p38g and mTOR activation and cardiac hypertrophy in MKK6-deficient hearts.

(A) Heart weight to tibia length ratio in 9-week-old MCK-Cre (n=8), Mkk6^MCK-KO (n=10) and Mkk3/6^MCK-KO (n=10). 1-way ANOVA followed by Tukey’s multiple comparison test. *P<0.05; ns: non-significant. Data are mean± SEM. (B) Immunoblot analysis of p38g phosphorylation and protein levels in heart lysates from 9-week-old Mkk6^MCK-KO and Mkk3/6^MCK-KO mice. (C) Immunoblot analysis of mTOR pathway activation by phosphorylation evaluation of its downstream target S6 in hearts from 9-week-old Mkk6^MCK-KO and Mkk3/6^MCK-KO mice.