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. 2022 Aug 16;11:e73792. doi: 10.7554/eLife.73792

Figure 1. Zika virus (ZIKV) infection induces the gasdermin E (GSDME)-mediated pyroptosis in JEG-3 cells.

JEG-3 or GSDME-KO JEG-3 cells were infected with ZIKV at a multiplicity of infection (MOI) of 1. At indicated time post-infection, cells were subjected to microscopy, cytotoxicity, and Western blot analyses. (A) JEG-3 cells were infected with ZIKV for 24 hr. Representative cell morphology was shown. Scale bar, 50 μm. (B) LDH levels in supernatant and cell viability were measured at indicated time post-infection (n=3). (C) Immunoblot analyses of GSDME-FL, GSDME-N, and GSDMD-FL in ZIKV-infected JEG-3 cells at indicated time post-infection. (D–F) JEG-3 and GSDME-KO JEG-3 cells were infected with ZIKV for 24 hr. Immunoblot analyses of GSDME-FL and GSDME-N by Western blot (D). Representative cell morphology was shown. Scale bar, 50 μm (E). LDH levels in supernatant and cell viability were measured (n=3) (F). LDH release (B, F) is presented as the mean ± SEM of three independent experiments, and the two-tailed unpaired Student’s t-test was used to calculate significance. ***, p<0,001; ****, p<0.0001; ns, no significance.

Figure 1—source data 1. Raw data for Figure 1.

Figure 1.

Figure 1—figure supplement 1. Gasdermin E (GSDME) does not affect Zika virus (ZIKV) replication in JEG-3 cells.

Figure 1—figure supplement 1.

Negative control (NC), GSDME KO, GSDME-overexpressed and GFP-overexpressed JEG-3 cells were infected with ZIKV at a multiplicity of infection (MOI) of 0.1. The supernatants of ZIKV-infected cells were harvested at 12–72 hr post-infection for plaque assay, and the titration was performed on Vero cells (n=3).
Figure 1—figure supplement 1—source data 1. Raw data for Figure 1—figure supplement 1.