FIG. 4.

PrlA3 and SecE(S120C) cross-linking inhibits the incorporation of the radioactive methionine into polypeptide. At 0 min, arabinose was added to the strains with the following genotypes: secE(S120C) prlA3 (squares), secE(S120C) secY⁺ (diamonds), and secE⁺ prlA3 (circles). At various time points samples were removed, and the ability of cells to incorporate [³⁵S]methionine into polypeptide was measured by pulse labeling followed by TCA precipitation and radioactivity measurement as described in Materials and Methods.