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Cysteine scanning for arabinose-dependent synthetic phenotypesa
| Form of secE | Diam (mm) of zone of growth inhibition with secY
|
|||
|---|---|---|---|---|
| Wild type | F64C | F67C | G69C | |
| Wild type | 0 | 0 | 0 | 0 |
| L118C | 0 | 0 | 0 | 0 |
| S120C | 0 | 0 | 24 | 0 |
| F121C | 0 | 0 | 0 | 0 |
| I122C | 0 | 0 | 0 | 0 |
| G124C | 0 | 25 | 0 | 0 |
Arabinose sensitivity assays are described in Materials and Methods. The position of the cysteine point mutation for each allele of secE and secY is indicated. Zero indicates no growth inhibition.
