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. 2022 Mar 29;20(7):1047–1060. doi: 10.1158/1541-7786.MCR-21-0477

Figure 3.

Figure 3. VPRBP knockdown leads to reduced cell proliferation and p53 stabilization. A, LNCaP cells were transfected with VPRBP siRNA and cells were harvested 3 days posttransfection to detect mRNA expression of VPRBP, OGT, p53, and p21 (n = 4). B, Effect of VPRBP knockdown on LNCaP cell proliferation was assessed by cell counting 5 days posttransfection; the cells were grown in the presence (CM) and absence of androgens (ADM; n = 4). C, Effect of VPRBP knockdown on LNCaP p53, markers of cell cycle and other proteins of interest was assessed by immunoblot analysis of cell lysate prepared 3 days posttransfection. D, Effect of VPRBP knockdown on cell proliferation was assessed by cell counting in TP53 knockout (KO) LNCaP cells grown in the presence (CM) and absence of androgens (ADM; n = 4). E, Effect of VPRBP knockdown on LNCaP versus TP53-KO LNCaP proteins of interest was assessed by immunoblot analysis. F, Effect of VPRBP knockdown on cell proliferation was assessed by cell counting 5 days posttransfection in VCaP cells grown in the presence (CM) and absence of androgens (ADM; n = 3). G, Effect of VPRBP knockdown on VCaP proteins of interest was assessed by immunoblot analysis. Results are expressed as means ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.00. Statistical analyses were performed by Student t test for qPCR and one-way ANOVA followed by Tukey post hoc analysis for cell proliferation.

VPRBP knockdown leads to reduced cell proliferation and p53 stabilization. A, LNCaP cells were transfected with VPRBP siRNA and cells were harvested 3 days posttransfection to detect mRNA expression of VPRBP, OGT, p53, and p21 (n = 4). B, Effect of VPRBP knockdown on LNCaP cell proliferation was assessed by cell counting 5 days posttransfection; the cells were grown in the presence (CM) and absence of androgens (ADM; n = 4). C, Effect of VPRBP knockdown on LNCaP p53, markers of cell cycle and other proteins of interest was assessed by immunoblot analysis of cell lysate prepared 3 days posttransfection. D, Effect of VPRBP knockdown on cell proliferation was assessed by cell counting in TP53 knockout (KO) LNCaP cells grown in the presence (CM) and absence of androgens (ADM; n = 4). E, Effect of VPRBP knockdown on LNCaP versus TP53-KO LNCaP proteins of interest was assessed by immunoblot analysis. F, Effect of VPRBP knockdown on cell proliferation was assessed by cell counting 5 days posttransfection in VCaP cells grown in the presence (CM) and absence of androgens (ADM; n = 3). G, Effect of VPRBP knockdown on VCaP proteins of interest was assessed by immunoblot analysis. Results are expressed as means ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Statistical analyses were performed by Student t test for qPCR and one-way ANOVA followed by Tukey post hoc analysis for cell proliferation.