REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
phosphorylated RET-Y905	Cell Signaling Technology	Cat#3221; RRID: AB_2179887
phosphorylated RET-Y1062	Abcam	Cat#ab51103; RRID: AB_870738
RET E1N8X	Cell Signaling Technology	Cat#14556; RRID: AB_2798509
RET	Abcam	Ab134100; RRID: AB_2920824
phosphorylated AKT-S473	Cell Signaling Technology	Cat#9271; RRID: AB_329825
AKT	Cell Signaling Technology	Cat#9272; RRID: AB_329827
phosphorylated ERK T202/Y204	Cell Signaling Technology	Cat#4377; RRID: AB_331775
ERK1/ERK2	Cell Signaling Technology	Cat#4695; RRID: AB_390779
GRB2	Cell Signaling Technology	Cat#36344; RRID: AB_2920901
PARP	Cell Signaling Technology	Cat#9542; RRID: AB_2160739
β-actin	Cell Signaling Technology	Cat#3700; RRID: AB_2242334
α-tubulin	Cell Signaling Technology	Cat#3873; RRID: AB_1904178
Purified anti-HA.11 Epitope Tag Antibody	BioLegend	Cat#901501; RRID: AB_2565006
Anti-Myc/c-Myc Antibody (9E10)	Santa Cruz Biotechnology	Cat#sc-40; RRID: AB_2857941
Na,K-ATPase α1 (D4Y7E) Rabbit mAb	Cell Signaling Technology	Cat#23565; RRID: AB_2798866
Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488	Invitrogen	Cat#A-11001; RRID: AB_2534069
Alexa Fluor™ 647 Goat Anti-Rabbit SFX Kit, highly cross-adsorbed	Invitrogen	Cat#A31634
Anti-Ret Antibody (8D10C9)	Santa Cruz Biotechnology	Cat#sc-101422; RRID: AB_2269605
Bacterial and virus strains
ONE SHOT STBL3 COMP E COLI	Invitrogen	Cat#C737303
Biological samples
Human tumor samples	This paper	N/A
Chemicals, peptides, and recombinant proteins
RPMI (cell medium)	Corning	Cat#10040-CV
Fetal Bovine Serum	Gibco	Cat#26140079
DAPI (4′,6-Diamidino-2-Phenylindole, Dilactate)	Thermo Fisher Scientific	Cat#EN62248
TrypLE Select, 10×	Thermo Fisher Scientific	Cat#A1217701
Hygromycin B	Invitrogen	Cat# 10687010
Puromycin dihydrochloride	Gibco	Cat#A1113803
Blasticidin	Gibco	Cat#R21001
Selpercatinib	ChemieTek	Cat#CT-LX292
Pralsetinib	ChemieTek	Cat#CT-BLU667
Human GDNF (glial-derived neurotrophic factor)	Preprotech	Cat#450-10-100UG
Recombinant Murine IL-3	Preprotech	Cat#213-13
Critical commercial assays
NEBNext® Ultra TM RNA Library Prep Kit for Illumina	New England BioLabs	Cat#E7760L
High-Capacity cDNA Reverse Transcription Kit	Applied Biosystems	Cat#4368814
Phusion High-Fidelity PCR Master Mix with GC Buffer	Thermo Fisher Scientific	Cat#F532L
iQ SYBR Green Supermix	Bio-Rad	Cat#1708880
Deposited data
RET::GRB2 ORF sequence	This paper	NCBI-Bankit (accession number: ON799207)
Experimental models: Cell lines
293FT Cell Line	Thermofisher/Invitrogen	Cat#R70007
SH-SY5Y	ATCC	Cat#CRL-2266
Ba/F3	Creative Biogene	Cat#CSC-RO0120
Oligonucleotides
Primer: RET-GRB2 long isoform_F Forward: TTGCGGACATCAGCAAAG	This paper	N/A
Primer: RET-GRB2 long isoform_F Reverse: CAGGAGCGCTCTCACTCTCT	This paper	N/A
Primer: RET-GRB2 short isoform_F Forward: TGCGGACATCAGCAAAGAC	This paper	N/A
Primer: RET-GRB2 short isoform_R Reverse: GAACTTCACCACCCAGAGG A	This paper	N/A
Primer RETe4F TGGTGATGGTGCCCTTCC	This paper	N/A
Primer RETe5R CTGATGCAGGTACCACGTCT	This paper	N/A
Primer: RET_e8_cDNA_F Forward: GGCTGGAGTGTGAGGAGTGT	This paper	N/A
Primer: RET_e9_cDNA_R Reverse: AGGTCTTGGTGCTGGGAGA G	This paper	N/A
Primer: RET_e19_cDNA_F Forward: CTGGTGGACTGTAATAATGC	This paper	N/A
Primer: RET_e20_cDNA_R Reverse: TTGGATATCTTGGAAACCCA	This paper	N/A
Recombinant DNA
pLV[Exp]-Puro-EF1A>3x N-flag-RET_1-18_GRB2_2-3-4-5	This paper	Custom order-By VectorBuilder VB210223-1137nqa
pLV[Exp]-Hygro- EF1A>hRET[NM_020975.6]/Myc	This paper	Custom order-By VectorBuilder VB210227-1059nqh
pLV[Exp]-Bsd- EF1A>hRET[NM_020630.6]/Myc	This paper	Custom order-By VectorBuilder VB210227-1062nya
pLV[Exp]-Puro-EF1A>[C-HA- RET_1-18-GRB2_2-3-4-5]/HA	This paper	Custom order-By VectorBuilder VB210227-1065xvg
pLV[Exp]-EGFP:T2A:Puro- EF1A>mCherry	VectorBuilder Inc	By VectorBuilder VB160109-10005
pLV[Exp]-Puro- EF1A>[C-HA-RET_1-18-GRB2_2-3-4-5]/HA	This paper	Custom order-By VectorBuilder VB210227-1065xvg
psPAX2	Addgene	Addgene #12260; RRID:Addgene_12260
pMD2.G	Addgene	Addgene #12259; RRID:Addgene_12259
pLV-RET51-c-Myc-C634R	This paper	N/A
pLV-RET51-c-Myc-K758M	This paper	N/A
pLV-RET9-c-Myc-D1014X	This paper	N/A
pLV-RETGRB2-K758M-HA	This paper	N/A
Software and algorithms
Spliced Transcripts Alignment to a Reference (STAR)	Dobin et al., 201326	http://code.google.com/p/rna-star/
HTSeq v.0.6.1	Putri et al., 202127	http://www-huber.embl.de/HTSeq
Gene Set Enrichment Analysis (GSEA)	Subramanian et al., 200528	https://www.gsea-msigdb.org/gsea/index.jsp
ImageJ	Abramoff et al., 201229	https://imagej.nih.gov/ij/
MiSeq	Illumina	https://www.illumina.com/systems/sequencing-platforms/miseq.html