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. 2022 Aug 16;12:13885. doi: 10.1038/s41598-022-18297-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Role of Pin in pyroptosis depends on Nrf2 in oxLDL-challenged HUVECs. After transfection with con-siRNA or Nrf2-siRNA, HUVECs were pretreated with or without 20 µM Pin for 2 h, and then incubated with 100 mg/L oxLDL for an additional 24 h. (a) TUNEL assay was used to observe cell DNA damage. Red: DNA damaged cells; Blue: nuclei. Quantitative determination of TUNEL-positive cells is shown in the graphs. Scale bar: 50 µm; (b) WB assays showing protein levels of pyroptosis-related markers. (c) Typical immunofluorescence images showing Caspase-1 expression in the cytoplasmic region. Green: Caspase-1; Blue: DAPI. Scale bar = 50 µm. (d,e) Showing IL-1β and IL-18 levels in the media measured by ELISA. Data are described with mean ± SD of at least 3 different experiments. *P < 0.05; **P < 0.01 vs. control cells; ^#P < 0.05; ^##P < 0.01 vs. cells challenged by oxLDL. ^{^}P < 0.05, ^{^^}P < 0.01 compared between the marked groups.