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. 2022 Aug 16;12:13885. doi: 10.1038/s41598-022-18297-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Role of Pin in the expressions of pyroptosis-related molecules and Nrf2/HO-1 axis in Apoe^–/– mice. The immunofluorescence stained images and quantitative analysis of CD31-NLRP3 and CD31-Caspase-1 positive cells (white arrow) in the aortic roots were displayed on (a,b). Red: CD31; Green: NLRP3 and Caspase-1; Blue: nuclei. Scale bar = 20 µm. (c) Showing IL-1β and IL-18 levels in the serum, assayed through ELISA. WB assay showing the protein expression levels of pyroptosis-related (d) and Nrf2/HO-1 axis-related molecules (e) in the aortic arches. The data are described as the mean ± SD of at least 3 different experiments. *P < 0.05; **P < 0.01 vs. model group.