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. 2022 Aug 16;13:4822. doi: 10.1038/s41467-022-32401-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a qPCR analysis of IFNB1 and IFIT1 mRNAs in IRF8 knockout (IRF8-KO-1 and IRF8-KO-2) or control THP-1 cells (transduced with the lenti-CRISPR-V2 containing a control gRNA) infected with SeV or HSV-1 for the indicated times. The knockout efficiencies of IRF8 were shown by immunoblots. b qPCR analysis of mRNA levels of the indicated genes in IRF8-KO or control THP-1 cells transfected with the indicated nucleic acids for 4 h. qPCR analysis of Ifnb1 and Cxcl10 mRNAs (c) and ELISA analysis of Ifn-β secretion (d) in WT, Irf8^−/− and Irf3^−/− BMDMs left uninfected or infected with the indicated viruses for 4 h. e Heat map of hierarchical clustering analysis of RNA-sequencing data from WT and Irf8^−/− BMDMs left uninfected or infected with HSV-1 for 4 h. The scale (log10) represents the changes in gene expression among different samples. qPCR analysis of Ifnb1 and Cxcl10 mRNAs (f) and ELISA analysis of Ifn-β secretion (g) in WT, Irf8^−/− and Irf3^−/− BMDMs transfected with the indicated nucleic acids for 4 h. Data in a–d, f and g are presented as mean. n = 2 technical replicates. Data were analyzed by unpaired two-tailed Student’s t-test. Source data are provided as a Source data file.