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. 2022 Aug 16;13:4822. doi: 10.1038/s41467-022-32401-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Coimmunoprecipitation of IRF8 and STING SAVI mutants in HEK293T cells. Cells were transfected with the indicated plasmids for 20 h followed by coimmunoprecipitation and immunoblotting analysis with the indicated antibodies. b Profiling analysis of IRF8 expression in healthy donors and patients with autoimmune diseases. Graph shows mean ± SEM, n = 5–15 independent samples. Data were analyzed by unpaired two-tailed Student’s t-test. c Immunoblotting analysis of IRF8^S151 and STING^S366 in PBMCs from healthy donors (n = 11 independent samples) and SLE patients (n = 10 independent samples). Relative phosphorylation abundance of IRF8^S151 and STING^S366 relative to β-actin was quantitated. Data were analyzed by unpaired two-tailed Student’s t test. d qPCR analysis of Ifnb1, Cxcl10, Il6 and Il1b mRNA levels in WT or Trex1^−/− BMDMs and BMDCs with or without additional Irf8 knockout by CRISP-Cas9. Data are presented as mean, n = 2 technical repeats. Data were analyzed by unpaired two-tailed Student’s t test. Source data are provided as a Source data file.