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. 2022 Aug 16;12(8):118. doi: 10.1038/s41408-022-00716-3

Fig. 4. Pretreatment or co-treatment with GSIs further augmented autologous patient MM cell lysis induced by BCMAxCD3 BisAbs.

Fig. 4

BMMCs of MM patients (A, n = 6; B, n = 5; C, n = 8) were pretreated (A, 4 h and 1 day; B, 1 day) or co-treated (C) with GSIs (2 nM LY-411575 or 1 µM DAPT). Indicated BisAbs (1, 10 nM in A, B; 1 nM in C) were added with PBMCs from the paired individuals for 1 day (A, C) or 4 days (C). Quantitative FC analysis was used to determine % patient MM cell (CD138+) depletion (A, B; C, left panel) and CD107a degranulation in autologous CD3+ patient T cells (C, right panel). Experiments were done in triplicate for each condition using multiple patient samples. Data are presented as means ± SDs (error bars). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns, not significant.