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. 2022 Aug 16;12(8):118. doi: 10.1038/s41408-022-00716-3

Fig. 6. LY-411575 minimally affected PL33-induced transient expression of checkpoint and co-stimulatory markers, as well as time-dependent increases in memory cell differentiation and CD8/CD4 ratios in patient T cells in longer 7 day-co-cultures.

Fig. 6

A Mixtures of patient T cells (n = 8) with target MM1S cells (E:T = 1:1) were treated with 1 nM PL33 in control (ctrl, black) or LY-411575 (2 nM, blue)-containing media on day 0 (d0). In 7 day time course expression studies, quantitative FC analysis was used to determine serial changes of immune checkpoint markers (PD1, TIM3, LAG3, TIGIT) and co-stimulatory markers (4-1BB, CD28) on CD8 (left in each marker panel) and CD4 (right in each marker panel) T cells. Shown is the relative expression of each marker by MFI ratios, which was normalized to the MFI values of ctrl groups on d0. The serial changes of each marker were analyzed by Wilcoxon matched pairs signed rank test. Different symbols represent T cells from different individuals. B Indicated MM target cells (n = 3) were co-cultured with patient T cells for 7 day (n = 8–11) in the presence of 1 nM PL33, in ctrl or LY-411575-containing media. Shown are % central memory (CM, CD45RA−CD62L+) and % EM (CD45RA−CD62L−), as well as CD8/CD4 ratios. Eight to 11 independent experiments were done in triplicates at each time point. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns, not significant.