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. 2022 Aug 16;13(8):713. doi: 10.1038/s41419-022-05158-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Schematic diagram of iPSC differentiation into hepatocytes. B Real-time PCR analysis for the mRNA expression of FOXA2, AFP, and ALB during hepatocyte differentiation. Data are presented as the fold change relative to day 0 of differentiation (n = 3). C Western blot analysis of FOXA2 expression levels during various stages of differentiation of iPSCs into hepatocytes. D Immunostaining of FOXA2 with the hepatic progenitor (HP) and mature hepatocyte (MH) markers, AFP and ALB, at the end of HP and MH stages, respectively. Western blotting (E) and immunofluorescence (F) analyses confirming the absence of FOXA2 protein in HP and MH derived from FOXA2^−/− iPSCs. DE definitive endoderm, PFG posterior foregut, HP hepatic progenitor, MH mature hepatocytes. The data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.