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. 2022 Aug 16;13(8):713. doi: 10.1038/s41419-022-05158-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A RT-qPCR analysis showing the mRNA expression of hepatic progenitor (HP) markers, FOXA1, FOXA3, ONECUT1, ONECUT2, ONECUT3, HNF1B, HNF1A, PROX1, HHEX, TBX3, CEBPA, and AFP in FOXA2^−/− HP relative to wild type (WT) controls (n = 6). B Immunofluorescence images showing the expression of CEBPA in HP derived from WT-iPSCs and FOXA2^−/−iPSCs. C Western blot analysis showing the upregulation of AFP in HP lacking FOXA2 compared to WT controls. D Immunofluorescence analysis showing co-expression of AFP and HNF4A in HP derived from WT-iPSCs and FOXA2^−/−iPSCs. Note the pattern of AFP expression in FOXA2^−/−HP. E RT-qPCR analysis showing the mRNA expression of mature hepatocyte (MH) markers, FOXA1, FOXA3, HNF4A, AAT, TTR, CPS1, CYP3A4, CYP26A1, and ALB in FOXA2^−/− MH relative to WT controls (n = 6). F Western blotting showing the upregulation of ALB protein in FOXA2^−/− MH compared to WT. G Immunofluorescence showing ALB protein expression in FOXA2^−/−MH compared to WT controls. Note the ALB distribution around cytoplasmic vacuoles. Nuclei were counterstained with Hoechst. The data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.