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. 2022 Aug 16;13(8):713. doi: 10.1038/s41419-022-05158-0

Fig. 7. FOXA2 overexpression rescues developmental and functional defects of iPSC-derived hepatocytes lacking FOXA2.

Fig. 7

A RT-qPCR analysis for the expression of hepatic progenitor markers, FOXA2, PROX1, ONECUT1, ONECUT1, HNF1B, HHEX, HNF1A, BMP4, CEBPA, and AFP in HP derived from FOXA2−/−iPSCs and WT-iPSCs, 48 h after FOXA2 overexpression (n = 3). B RT-qPCR analysis for the expression of ER stress markers, CHOP, BiP, DP5, and IL6 in HP derived from FOXA2−/−iPSCs and WT-iPSCs, 48 h after FOXA2 overexpression (n = 3). C ALBUMIN (ALB) ELISA assay showing ALB levels measured in culture media of mature hepatocytes (MH) derived from FOXA2−/−iPSCs, transfected with FOXA2 plasmid or empty vector (Lacz) at hepatic progenitor stage (n = 5). Quantification of Oil Red O staining (D) (n = 4), glycerol levels (n = 3) (E), CYP3A4 activity (F) (n = 6), glucose uptake (n = 3) (G), and glycogen accumulation n = 3) (H) in MH derived from FOXA2−/−iPSCs and WT-iPSCs, transfected with FOXA2 plasmid or empty vector at HP stage. Data are represented as mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.001.