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. 2022 Aug 16;12:13859. doi: 10.1038/s41598-022-18075-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Real-time PCR results. (a) Collagen type I (COL1) and (b) alkaline phosphatase (ALP) genes expression of 3D bone tissue models cultured for 14 days under static culture (control), bi-directional perfusion, or bi-directional perfusion combined with PEMF stimulation (p < 0.05 with respect to control indicated by #, p < 0.05 with respect to bi-directional perfusion indicated by §). Bars represent means and standard deviations, replicates n = 3.