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. 2022 May 21;127(4):766–775. doi: 10.1038/s41416-022-01842-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Receiver Operating Characteristic (ROC) curve resulting from a four-fold cross-validation using 32 positive and 32 negative cases. The area under the curve (AUC) and the True positive rate (TPR) are reported. b ROC plot of the classifier when tested on 161 cases, with a full range of tumour regression grading in percent (TRG 10–100%) and c precision recall curve. d Heatmap visualisation of the expression of the 21-transcript signature in 161 pretreatment biopsies of rectal cancer patients.