Fig. 2.

Sp1 activates DDX39B transcription in CRC cells. a HCT116 cells were transiently transfected with the plasmids of ETS-1, Sp1, or c-JUN, respectively. The mRNA and protein levels of DDX39B were measured by qPCR and western blotting. b HCT116 cells were transiently transfected with siRNAs targeting ETS-1, Sp1, or c-JUN, respectively. The mRNA and protein expressions of DDX39B were determined by qRT-PCR and western blotting. c The relative luciferase activity was detected in HCT116 cells transfected with the indicated DDX39B promoter in the presence or absence of Sp1. d DNA fragments from HCT116 cells were immunoprecipitated with the Sp1-specific antibody and analyzed by qPCR using the indicated primers. e The correlations of transcript levels between DDX39B and Sp1 in human colon and rectal adenocarcinoma tissues were analyzed through an online tool (https://xenabrowser.net/). Data are presented as mean ± SD. The p values were obtained by two-tailed unpaired t test (a) or one-way ANOVA (b–d). *p < 0.05, ***p < 0.001, ns not significant