TABLE 3.
GUS activity for P. syringae pv. syringae FF5 and FF5.32 containing various promoter constructs with the algD upstream region
| Straina | GUS activity (U/mg of protein)b in strains containing:
|
||
|---|---|---|---|
| pSK3 | pSK4 | pRG960sd | |
| FF5(pPSR12) | 537a | 88b | 66b |
| FF5.32 | 398a | 82b | 64b |
a
FF5(pPSR12) is the wild type, and FF5.32 is an algR1 mutant derived from FF5(pPSR12).
b
Mean values followed by the same letter are not significantly different at P = 0.05 by the Student-Newman Keuls test. pSK3 contains the algD promoter in the transcriptionally active orientation, pSK4 contains algD in the transcriptionally inactive orientation, and pRG960sd is the vector used for construction of pSK3 and pSK4. FF5(pPSR12, pSK3) and FF5.32(pRG960sd) were regarded as positive and negative controls for the GUS assay, respectively.