Fig. 7.

Loss of C9orf72 affects synaptic vesicle pools in the hippocampus in vivo. a Representative transmission electron micrographs of the CA3 region of the hippocampus from 12-week old C9orf72-WT or C9orf72-KO mice. Scale bar 0.5 μm. Box denotes zoomed area. An excitatory nerve terminal is outlined by the red line in the zoomed area. Scale bar 0.15 μm. b Quantification of the density of synaptic vesicles (SV density, μm²) and the number of docked synaptic vesicles per synapse (Docked SVs/synapse) in C9orf72-WT and -KO excitatory synapses. Data are is presented as box and whisker plots; n (synapses analysed) WT = 81, KO = 105 obtained from 4 C9orf72-WT and 5 C9orf72-KO animals. Statistical significance was determined by unpaired two-tailed t test, **P < 0.01, ****P < 0.0001