Table 1.
Primary antibodies used in this study
| Antibody | Host | Vendor information | Application | Comments |
|---|---|---|---|---|
| Actin | Mouse | MAB 1501, Merck Life Science UK Ltd, Gillingham, UK | WB: 1:5000 | |
| C9orf72 | Mouse | GTX632041, GeneTex, Insight Biotechnology Ltd, London, UK |
IP: 4 µg IF and PLA: 1:500 IHC human: 1:1600 |
KO verified (Supplier) [42] Used to detect endogenous C9orf72 protein in Fig. 1d, e (IP); Fig. 1f, Supplementary Fig. 4, Online Resource (IF, PLA); Fig. 6 (IHC human) |
| C9orf72 | Rabbit | 25757-1-AB, Proteintech Europe Ltd, Manchester, UK | WB: 1:1000 |
KO verified (Supplier and Fig. 1e, Supplementary Fig. 1c, Supplementary Fig. 2a, Online Resource) Used to detect endogenous C9orf72 protein in Fig. 1d, e, Supplementary Fig. 1a, Supplementary Fig. 2a, Supplementary Fig. 4c, Supplementary Fig. 6b, Supplementary Fig. 9b Online Resource (WB) |
| C9orf72 | Rabbit | sc-138763, Santa Cruz Biotechnology Inc., Heidelberg, Germany | WB: 1:500 | Used to detect overexpressed Myc-tagged C9orf72 in Fig. 1a, b |
| FLAG | Mouse | Clone M2, F3165, Sigma-Aldrich/Merck Life Science UK Ltd | IP: 4 µg | |
| GAPDH | Rabbit | 14C10, Cell Signalling Technology Europe BV, Leiden, Netherlands | WB: 1:2000 | |
| Gephyrin | Mouse | 147011, Synaptic Systems GmbH, Goettingen, Germany | IF: 1:500 | KO verified (Supplier) |
| GFP | Mouse | Clone JL-8, 632381, Clontech, Takara Bio Europe S.A.S |
WB: 1:10,000 IP: 1 µg |
|
| GFP | Rabbit | ab6556, Abcam Plc, Cambridge, UK | WB: 1:2500 | |
| Homer I | Rabbit | 160002, Synaptic Systems | IF: 1:500 | |
| Iba1 | Goat | ab5076, Abcam | IF: 1:750 | |
| MAP2 | Chicken | NB300-213, Novus Biologicals, Bio-Techne Ltd, Oxon, UK | IF: 1:1000 | |
| Myc | Mouse | clone 9B11, 2276S, Cell Signalling |
WB: 1:2000 IF: 1:2000 IP: 0.318 µg |
|
| Myc | Rabbit | ab9106, Abcam, | WB: 1:2000 | |
| PDI | Mouse | GTX25484, GeneTex | WB: 1:2000 | |
| PSD95 | Mouse | Clone 6G6-1C9, MAB 1596, Merck Life Science UK Ltd | IF: 1:1000 | Validated by relative expression in mouse brain v mouse liver and skeletal muscle (Supplier) |
| PSD95 | Goat | ab12093, Abcam | IHC mouse 1:100 | |
| SMCR8 | Rabbit | GTX635798, GeneTex | WB: 1:1000 | |
| SSRP1 | Mouse | ab26212, Abcam | WB: 1:500 | |
| Synaptophysin 1 | Chicken | 101006, synaptic systems | IF: 1:500 | |
| Synapsin 1 | Mouse | 106001 and 106011, synaptic systems |
WB: 1:1000 IF: 1:500 IHC mouse: 1:200 IHC human 1:3200 |
KO verified (Supplier) Used in Fig. 3 (IF), Figs. 4, 5, Supplementary Fig. 8b, Online Resource (IHC mouse), Fig. 6 (IHC human), Supplementary Fig. 6c, Supplementary Fig. 8a, Online Resource (WB) |
| Synapsin 1 | Rabbit | S193, Sigma-Aldrich/Merck | IF and PLA: 1:2000 | Used in Fig. 1f, Supplementary Fig. 4, 5, Online Resource (IF and PLA) |
| Synapsin 3 | Rabbit | 106303, Synaptic Systems |
WB: 1:1000 IF and PLA: 1:500 IHC mouse 1:500 |
KO verified (Supplier) |
| SV2 | Mouse | SV2, Developmental Studies Hybridoma Bank (DSHB), University of Iowa, IA, USA |
IF: 1:1000 IHC: 1:200 WB: 1:1000 |
KO verified [16] |
| VGAT | Rabbit | 131003, Synaptic Systems | IF: 1:500 | KO verified (Supplier) |
| Vimentin | Chicken | AB5733, Merck Life Science UK Ltd | IF: 1:1000 | |
| V5 | Rabbit | Clone D3H8Q, 13202, Cell Signalling | WB: 1:500 | |
| V5 | Mouse | MA5-15253, Thermo Fisher Scientific, Runcorn, UK |
WB: 1:1000 IF: 1:1000 |