Figure 1.

Graphic illustration of experimental schedule. (A) Mice were randomly divided into four groups. (B) Six or three-week-old ICR mice were pre-treated with i.p. injections of Mel or Sal once daily (at 24 h intervals) for four consecutive days before achieving Cyc injection, and for 14 additional days during chemotherapy treatment. On Day 4, mice were injected with Sal or Cyc (75 mg/kg). Blood and/or ovaries were collected 12 h or two weeks after Cyc injection. Fertility assessment was performed 115 days after Cyc injection. (C) Three-week-old prepubertal female mice were injected i.p. with 75 mg/kg body weight Cyc 12 h after injection of PMSG. Mice were sacrificed 8, 12, 24, or 36 h after chemotherapy treatment and their ovaries were excised (n = 4). (D) Prepubertal female mice were injected i.p. with 75 mg/kg body weight Cyc 24 h after injection of PMSG. Mice were sacrificed 12, 24, or 36 h after chemotherapy treatment and their ovaries were excised.