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. 2022 Aug 3;13:895095. doi: 10.3389/fendo.2022.895095

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Copyright © 2022 Feng, Ma, Li, Pei, Deng, Jia and Ma

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of Mel on expression of AMH and oxidative stress in ovaries of six-week-old female mice 14 days after Cyc chemotherapy (Day 18). (A) AMH was expressed in granulosa cells of secondary and early antral follicles in Sal, Cyc, Mel, and Mel + Cyc groups (n = 6). Bar = 200 μm. (B) Cyc combined with Mel therapy prevented the decrease of serum AMH and LH levels but there was no significant change in serum FSH levels (n = 6). (C) Biochemical analysis of the ovarian CAT, SOD activities, and MDA levels (n = 6). (D) Phosphorylation levels of PTEN and FOXO3a did not change significantly after treatment with Cyc and Mel (n = 6). ^a,b Values with different letters are significantly different from each other.